Differential expression of Cosmc, T-synthase and mucins in Tn-positive colorectal cancers.

ABSTRACT:

Background

The Tn neoantigen (GalNAcα1-O-Ser/Thr) is an O-glycan expressed in various types of human cancers. Studies in several Tn-expressing cancer cell lines and pancreatic tumors have identified loss of Cosmc expression caused by either mutations or promoter hypermethylation. In this study, we explored the mechanism(s) for Tn expression in human colorectal cancers (CRC).

Methods

Tn-expressing cell populations were isolated from CRC cell lines by Fluorescence-associated cell sorting (FACS). The expression of the Tn and sialylated Tn (STn) antigens, Cosmc, T-synthase, and mucins was characterized in paired specimens with CRC and in CRC cell lines by immunostaining, western blot, and qPCR.

Results

Using well-defined monoclonal antibodies, we confirmed prevalent Tn/ST

SUBMITTER: Sun X 

PROVIDER: S-EPMC6097208 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature