Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome.

ABSTRACT:

Background

Numerous scaffold-level sequences for wheat are now being released and, in this context, we report on a strategy for improving the overall assembly to a level comparable to that of the human genome.

Results

Using chromosome 7A of wheat as a model, sequence-finished megabase-scale sections of this chromosome were established by combining a new independent assembly using a bacterial artificial chromosome (BAC)-based physical map, BAC pool paired-end sequencing, chromosome-arm-specific mate-pair sequencing and Bionano optical mapping with the International Wheat Genome Sequencing Consortium RefSeq v1.0 sequence and its underlying raw data. The combined assembly results in 18 super-scaffolds across the chromosome. The value of finished genome regions is demonstrated f

SUBMITTER: Keeble-Gagnere G 

PROVIDER: S-EPMC6097218 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature