In Vivo Imaging of the Retina, Choroid, and Optic Nerve Head in Guinea Pigs.

ABSTRACT:

Purpose

Guinea pigs are increasingly being used as a model of myopia, and may also represent a novel model of glaucoma. Here, optical coherence tomography (OCT) imaging was performed in guinea pigs. In vivo measurements of retinal, choroidal, and optic nerve head parameters were compared with histology, and repeatability and interocular variations were assessed.

Methods

OCT imaging and histology were performed on adult guinea pigs (n = 9). Using a custom program in MATLAB, total retina, ganglion cell/nerve fiber layer (GC/NFL), outer retina, and choroid thicknesses were determined. Additionally, Bruch's membrane opening (BMO) area and diameter, and minimum rim width were calculated. Intraobserver, interocular, and intersession coefficients of variation (CV) and intraclass co

SUBMITTER: Jnawali A 

PROVIDER: S-EPMC6097717 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature