Toward Cleavable Metabolic/pH Sensing "Double Agents" Hyperpolarized by NMR Signal Amplification by Reversible Exchange.

ABSTRACT: We show the simultaneous generation of hyperpolarized ¹³ C-labeled acetate and ¹⁵ N-labeled imidazole following spin-relay of hyperpolarization and hydrolysis of the acetyl moiety on 1-¹³ C-¹⁵ N₂ -acetylimidazole. Using SABRE-SHEATH (Signal Amplification by Reversible Exchange in SHield Enables Alignment Transfer to Heteronuclei), transfer of spin order occurs from parahydrogen to acetylimidazole ¹⁵ N atoms and the acetyl ¹³ C site (≈263-fold enhancement), giving rise to relatively long hyperpolarization lifetimes at 0.3 T (T₁ ≈52 s and ≈149 s for ¹³ C and ¹⁵ N, respectively). Immediately following polarization transfer, the ¹³ C-labeled acetyl group is hydrolytically cl

SUBMITTER: Kidd BE 

PROVIDER: S-EPMC6097920 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature