Analyses of the three 1-Cys Peroxiredoxins from Aspergillus fumigatus reveal that cytosolic Prx1 is central to H₂O₂ metabolism and virulence.

ABSTRACT: Standing among the front defense strategies against pathogens, host phagocytic cells release various oxidants. Therefore, pathogens have to cope with stressful conditions at the site of infection. Peroxiredoxins (Prx) are highly reactive and abundant peroxidases that can support virulence and persistence of pathogens in distinct hosts. Here, we revealed that the opportunistic human pathogen A. fumigatus presents three 1-Cys Prx (Prx6 subfamily), which is unprecedented. We showed that PrxB and PrxC were in mitochondria, while Prx1 was in cytosol. As observed for other Prxs, recombinant Prx1 and PrxC decomposed H₂O₂ at elevated velocities (rate constants in the 10⁷ M^-1s^-1 range). Deletion mutants for each Prx displayed higher sensitivity

SUBMITTER: Rocha MC 

PROVIDER: S-EPMC6098058 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature