Conditional control of fluorescent protein degradation by an auxin-dependent nanobody.

ABSTRACT: The conditional and reversible depletion of proteins by auxin-mediated degradation is a powerful tool to investigate protein functions in cells and whole organisms. However, its wider applications require fusing the auxin-inducible degron (AID) to individual target proteins. Thus, establishing the auxin system for multiple proteins can be challenging. Another approach for directed protein degradation are anti-GFP nanobodies, which can be applied to GFP stock collections that are readily available in different experimental models. Here, we combine the advantages of auxin and nanobody-based degradation technologies creating an AID-nanobody to degrade GFP-tagged proteins at different cellular structures in a conditional and reversible manner in human cells. We demonstrate efficient and revers

SUBMITTER: Daniel K 

PROVIDER: S-EPMC6098157 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature