Anacardic Acid Reduces Acetylation of H4K12 in Mouse Oocytes during Vitrification.

ABSTRACT:

Objective

Over the last years, vitrification has been widely used for oocyte cryopreservation, in animals and humans; however, it frequently causes minor and major epigenetic modifications. The effect of oocyte vitrification on levels of acetylation of histone H4 at lysine 12 (AcH4K12), and histone acetyltransferase (Hat) expression, was previously assessed; however, little is known about the inhibition of Hat expression during oocyte vitrification. This study evaluated the effect of anacardic acid (AA) as a Hat inhibitor on vitrified mouse oocytes.

Materials and methods

In this experimental study, 248 mouse oocytes at metaphase II (MII) stage were divided in three experimental groups namely, fresh control oocytes (which were not affected by vitrification), frozen/thawed ooc

SUBMITTER: Ghazifard A 

PROVIDER: S-EPMC6099150 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature