Peptidomic analysis of endogenous plasma peptides from patients with pancreatic neuroendocrine tumours.

ABSTRACT:

Rationale

Diagnosis of pancreatic neuroendocrine tumours requires the study of patient plasma with multiple immunoassays, using multiple aliquots of plasma. The application of mass spectrometry based techniques could reduce the cost and amount of plasma required for diagnosis.

Methods

Plasma samples from two patients with pancreatic neuroendocrine tumours were extracted using an established acetonitrile-based plasma peptide enrichment strategy. The circulating peptidome was characterised using nano and high flow rate liquid chromatography/mass spectrometry (LC/MS) analyses. To assess the diagnostic potential of the analytical approach, a large sample batch (68 plasmas) from control subjects, and aliquots from subjects harbouring two different types of pancreatic neuroendocri

SUBMITTER: Kay RG 

PROVIDER: S-EPMC6099210 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature