High V-PPase activity is beneficial under high salt loads, but detrimental without salinity.

ABSTRACT: The membrane-bound proton-pumping pyrophosphatase (V-PPase), together with the V-type H⁺ -ATPase, generates the proton motive force that drives vacuolar membrane solute transport. Transgenic plants constitutively overexpressing V-PPases were shown to have improved salinity tolerance, but the relative impact of increasing PP_i hydrolysis and proton-pumping functions has yet to be dissected. For a better understanding of the molecular processes underlying V-PPase-dependent salt tolerance, we transiently overexpressed the pyrophosphate-driven proton pump (NbVHP) in Nicotiana benthamiana leaves and studied its functional properties in relation to salt treatment by primarily using patch-clamp, impalement electrodes and pH imaging. NbVHP overexpression led to higher vacuolar

SUBMITTER: Graus D 

PROVIDER: S-EPMC6099232 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature