Evolution and diversification of the plant gibberellin receptor GID1.

ABSTRACT: The plant gibberellin (GA) receptor GID1 shows sequence similarity to carboxylesterase (CXE). Here, we report the molecular evolution of GID1 from establishment to functionally diverse forms in eudicots. By introducing 18 mutagenized rice GID1s into a rice gid1 null mutant, we identified the amino acids crucial for GID1 activity in planta. We focused on two amino acids facing the C2/C3 positions of ent-gibberellane, not shared by lycophytes and euphyllophytes, and found that adjustment of these residues resulted in increased GID1 affinity toward GA₄, new acceptance of GA₁ and GA₃ carrying C13-OH as bioactive ligands, and elimination of inactive GAs. These residues rendered the GA perception system more sophisticated. We conducted phylogenetic

SUBMITTER: Yoshida H 

PROVIDER: S-EPMC6099883 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature