Split-BioID - Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment.

ABSTRACT: To complement existing affinity purification (AP) approaches for the identification of protein-protein interactions (PPI), enzymes have been introduced that allow the proximity-dependent labeling of proteins in living cells. One such enzyme, BirA* (used in the BioID approach), mediates the biotinylation of proteins within a range of approximately 10 nm. Hence, when fused to a protein of interest and expressed in cells, it allows the labeling of proximal proteins in their native environment. As opposed to AP that relies on the purification of assembled protein complexes, BioID detects proteins that have been marked within cells no matter whether they are still interacting with the protein of interest when they are isolated. Since it biotinylates proximal proteins, one can moreover capitaliz

SUBMITTER: Schopp IM 

PROVIDER: S-EPMC6100705 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature