Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia.

ABSTRACT:

Background

In Klebsiella pneumoniae, mgrB and components of pmrHFIJKLM operon play a major role in colistin resistance.

Methods

We analyzed 23 nonduplicating colistin-resistant K. pneumoniae isolates, collected during the years 2011-2015, for the possible mechanism underlying their nonsusceptibility to colistin. Isolates were tested for their minimum inhibitory concentrations and antibiotic resistance determinants and genotyped by multilocus sequence typing (MLST). The MLST genes, antibiotic-resistant genes, and the genes of two component system (TCS), including mgrB, PhoQ/PhoP, pmrA/B, and CrrAB, were investigated by PCR amplification and Sanger sequencing.

Results

All isolates were distributed in eight sequence types (STs) and showed muta

SUBMITTER: Uz Zaman T 

PROVIDER: S-EPMC6101004 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature