Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation.

ABSTRACT: Genetically engineered mouse models (GEMMs) are extremely valuable in revealing novel biological insights into the initiation and progression mechanisms of human diseases such as cancer. Transgenic and conditional knockout mice have been frequently used for gene overexpression or ablation in specific tissues or cell types in vivo. However, generating germline mouse models can be time-consuming and costly. Recent advancements in gene editing technologies and the feasibility of delivering DNA plasmids by viral infection have enabled rapid generation of non-germline autochthonous mouse cancer models for several organs. The bladder is an organ that has been difficult for viral vectors to access, due to the presence of a glycosaminoglycan layer covering the urothelium. Here, we describe a novel

SUBMITTER: Yu C 

PROVIDER: S-EPMC6101102 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature