Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids.

ABSTRACT: Nature has a variety of possibilities to create new protein functions by modifying the sequence of the individual amino acid building blocks. However, all variations are based on the 20 canonical amino acids (cAAs). As a way to introduce additional physicochemical properties into polypeptides, the incorporation of non-canonical amino acids (ncAAs) is increasingly used in protein engineering. Due to their relatively short length, the modification of ribosomally synthesized and post-translationally modified peptides by ncAAs is particularly attractive. New functionalities and chemical handles can be generated by specific modifications of individual residues. The selective pressure incorporation (SPI) method utilizes auxotrophic host strains that are deprived of an essential amino acid in che

SUBMITTER: Nickling JH 

PROVIDER: S-EPMC6101111 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature