Lyophilized human cells stored at room temperature preserve multiple RNA species at excellent quality for RNA sequencing.

ABSTRACT: Biobanks operating at ambient temperatures would dramatically reduce the costs associated with standard cryogenic storage. In the present study, we used lyophilization to stabilize unfractionated human cells in a dried state at room temperature and tested the yield and integrity of the isolated RNA by microfluidic electrophoresis, RT-qPCR and RNA sequencing. RNA yields and integrity measures were not reduced for lyophilized cells (unstored, stored for two weeks or stored for two months) compared to their paired controls. The abundance of the selected mRNAs with various expression levels, as well as enhancer-associated RNAs and cancer biomarker long non-coding RNAs (MALAT1, GAS5 and TUG1), were not significantly different between the two groups as assessed by RT-qPCR. R

SUBMITTER: Ozgyin L 

PROVIDER: S-EPMC6101130 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature