Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex.

ABSTRACT:

Background and purpose

Candida parapsilosis is a common cause of candidemia in children and patients with onco-hematological diseases, septic arthritis, peritonitis, vaginitis, and nail and skin infections. Regarding this, the present study was condcuted to evaluate intra- and inter-species variation within beta-tubulin DNA sequence of C. parapsilosis complex in order to establish the utilization of this gene in the identification and phylogenetic analysis of the species.

Materials and methods

A total of 23 isolates representing three different species of C. parapsilosis complex were used in this study, all of which were identifed by ITS-sequencing. For the successful amplification of beta-tubulin gene, a newly designed set of pan-Candida primers

SUBMITTER: Kharazi M 

PROVIDER: S-EPMC6101151 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature