Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments.

ABSTRACT: The retina is a complex tissue that initiates and integrates the first steps of vision. Dysfunction of retinal cells is a hallmark of many blinding diseases, and future therapies hinge on fundamental understandings about how different retinal cells function normally. Gaining such information with biochemical methods has proven difficult because contributions of particular cell types are diminished in the retinal cell milieu. Live retinal imaging can provide a view of numerous biological processes on a subcellular level, thanks to a growing number of genetically encoded fluorescent biosensors. However, this technique has thus far been limited to tadpoles and zebrafish larvae, the outermost retinal layers of isolated retinas, or lower resolution imaging of retinas in live animals. Here we pr

SUBMITTER: Giarmarco MM 

PROVIDER: S-EPMC6101158 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature