Molecular mechanism of modulating arrestin conformation by GPCR phosphorylation.

ABSTRACT: Arrestins regulate the signaling of ligand-activated, phosphorylated G-protein-coupled receptors (GPCRs). Different patterns of receptor phosphorylation (phosphorylation barcode) can modulate arrestin conformations, resulting in distinct functional outcomes (for example, desensitization, internalization, and downstream signaling). However, the mechanism of arrestin activation and how distinct receptor phosphorylation patterns could induce different conformational changes on arrestin are not fully understood. We analyzed how each arrestin amino acid contributes to its different conformational states. We identified a conserved structural motif that restricts the mobility of the arrestin finger loop in the inactive state and appears to be regulated by receptor phosphorylation. Distal and prox

SUBMITTER: Sente A 

PROVIDER: S-EPMC6101189 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature