Changes in microRNA expression during differentiation of embryonic and induced pluripotent stem cells to definitive endoderm.

ABSTRACT: Pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have the potential to treat type 1 diabetes through cell replacement therapy. However, the protocols used to generate insulin-expressing cells in vitro frequently result in cells which have an immature phenotype and are functionally restricted. MicroRNAs (miRNAs) are now known to be important in cell fate specification, and a unique miRNA signature characterises pancreatic development at the definitive endoderm stage. Several studies have described differences in miRNA expression between ESCs and iPSCs. Here we have used microarray analysis both to identify miRNAs up- or down-regulated upon endoderm formation, and also miRNAs differentially expressed between ESCs and iPSCs. Several miR

SUBMITTER: Francis N 

PROVIDER: S-EPMC6101203 | biostudies-literature | 2015 Sep-Nov

REPOSITORIES: biostudies-literature