Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis.

ABSTRACT: We developed genetically encoded fluorescence resonance energy transfer (FRET)-based sensors that display a large ratiometric change upon Zn(2+) binding, have affinities that span the pico- to nanomolar range and can readily be targeted to subcellular organelles. Using this sensor toolbox we found that cytosolic Zn(2+) was buffered at 0.4 nM in pancreatic beta cells, and we found substantially higher Zn(2+) concentrations in insulin-containing secretory vesicles.

SUBMITTER: Vinkenborg JL 

PROVIDER: S-EPMC6101214 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature