Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia.

ABSTRACT: Intestinal ischemia remains a major cause of morbidity and mortality in human and veterinary patients. Many disease processes result in intestinal ischemia, when the blood supply and therefore oxygen is decreased to the intestine. This leads to intestinal barrier loss and damage to the underlying tissue. Intestinal stem cells reside at the base of the crypts of Lieberkühn and are responsible for intestinal renewal during homeostasis and following injury. Ex vivo cell culture techniques have allowed for the successful study of epithelial stem cell interactions by establishing culture conditions that support the growth of three-dimensional epithelial organ-like systems (termed "enteroids" and "colonoids" from the small and large intestine, respectively). These enteroids are composed of crypt

SUBMITTER: Stieler Stewart A 

PROVIDER: S-EPMC6101266 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature