Chromatin-associated APC regulates gene expression in collaboration with canonical WNT signaling and AP-1.

ABSTRACT: Mutation of the APC gene occurs in a high percentage of colorectal tumors and is a central event driving tumor initiation in the large intestine. The APC protein performs multiple tumor suppressor functions including negative regulation of the canonical WNT signaling pathway by both cytoplasmic and nuclear mechanisms. Published reports that APC interacts with β-catenin in the chromatin fraction to repress WNT-activated targets have raised the possibility that chromatin-associated APC participates more broadly in mechanisms of transcriptional control. This screening study has used chromatin immunoprecipitation and next-generation sequencing to identify APC-associated genomic regions in colon cancer cell lines. Initial target selection was performed by comparison and statistical analy

SUBMITTER: Hankey W 

PROVIDER: S-EPMC6101278 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature