Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells.

ABSTRACT: The hybridization capture of chromatin-associated proteins for proteomics (HyCCAPP) technology was initially developed to uncover novel DNA-protein interactions in yeast. It allows analysis of a target region of interest without the need for prior knowledge about likely proteins bound to the target region. This, in theory, allows HyCCAPP to be used to analyze any genomic region of interest, and it provides sufficient flexibility to work in different cell systems. This method is not meant to study binding sites of known transcription factors, a task better suited for Chromatin Immunoprecipitation (ChIP) and ChIP-like methods. The strength of HyCCAPP lies in its ability to explore DNA regions for which there is limited or no knowledge about the proteins bound to it. It can also be a convenie

SUBMITTER: Guillen-Ahlers H 

PROVIDER: S-EPMC6101452 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature