Characterization of Aquatic Biofilms with Flow Cytometry.

ABSTRACT: Biofilms are dynamic consortia of microorganism that play a key role in freshwater ecosystems. By changing their community structure, biofilms respond quickly to environmental changes and can be thus used as indicators of water quality. Currently, biofilm assessment is mostly based on integrative and functional endpoints, such as photosynthetic or respiratory activity, which do not provide information on the biofilm community structure. Flow cytometry and computational visualization offer an alternative, sensitive, and easy-to-use method for assessment of the community composition, particularly of the photoautotrophic part of freshwater biofilms. It requires only basic sample preparation, after which the entire sample is run through the flow cytometer. The single-cell optical and fluoresce

SUBMITTER: Sgier L 

PROVIDER: S-EPMC6101579 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature