Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions.

ABSTRACT: The three-dimensional organization of the genome is linked to its function. For example, regulatory elements such as transcriptional enhancers control the spatio-temporal expression of their target genes through physical contact, often bridging considerable (in some cases hundreds of kilobases) genomic distances and bypassing nearby genes. The human genome harbors an estimated one million enhancers, the vast majority of which have unknown gene targets. Assigning distal regulatory regions to their target genes is thus crucial to understand gene expression control. We developed Promoter Capture Hi-C (PCHi-C) to enable the genome-wide detection of distal promoter-interacting regions (PIRs), for all promoters in a single experiment. In PCHi-C, highly complex Hi-C libraries are specifically enr

SUBMITTER: Schoenfelder S 

PROVIDER: S-EPMC6102006 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature