Purification and Reconstitution of TRPV1 for Spectroscopic Analysis.

ABSTRACT: Polymodal ion channels transduce multiple stimuli of different natures into allosteric changes; these dynamic conformations are challenging to determine and remain largely unknown. With recent advances in single-particle cryo-electron microscopy (cryo-EM) shedding light on the structural features of agonist binding sites and the activation mechanism of several ion channels, the stage is set for an in-depth dynamic analysis of their gating mechanisms using spectroscopic approaches. Spectroscopic techniques such as electron paramagnetic resonance (EPR) and double electron-electron resonance (DEER) have been mainly restricted to the study of prokaryotic ion channels that can be purified in large quantities. The requirement for large amounts of functional and stable membrane proteins has hampe

SUBMITTER: Sierra-Valdez FJ 

PROVIDER: S-EPMC6102038 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature