Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology.

ABSTRACT: Peripheral nerves extend throughout the body, innervating target tissues with motor or sensory axons. Due to widespread distribution, peripheral nerves are frequently damaged because of trauma or disease. As methods and strategies have been developed to assess peripheral nerve injury in animal models, function and regeneration, analyzing the morphometry of the peripheral nerve has become an essential terminal outcome measurement. Toluidine blue staining of nerve cross sections obtained from resin embedded nerve sections is a reproducible method for qualitative and quantitative assessments of peripheral nerves, enabling visualization of morphology number of axons and degree of myelination. This technique, as with many other histological methods, can be difficult to learn and master using st

SUBMITTER: Ghnenis AB 

PROVIDER: S-EPMC6102041 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature