Full-length genome and molecular characterization of dengue virus serotype 2 isolated from an imported patient from Myanmar.

ABSTRACT:

Background

Dengue is the most common mosquito-borne infection worldwide and a serious threat to global public health. Sporadic dengue virus serotype 2 (DENV-2) imported cases from Myanmar have been documented almost every year in Yunnan Province of China since 2005. However, the complete genome sequences of DENV-2 isolates imported from Myanmar are not available.

Methods

The full-length genome of the DENV-2 strain (YNPE2), isolated from an imported case from Myanmar in 2013, was identified by the next-generation sequencing. The extreme ends of the viral genome were validated by 5'/3' RACE and Sanger sequencing. Furthermore, phylogenetic, recombination and selection pressure analyses were conducted for the molecular characterization of YNPE2 strain.

Results

Whole-genom

SUBMITTER: Zeng Z 

PROVIDER: S-EPMC6102819 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature