Mutant TP53 G245C and R273H promote cellular malignancy in esophageal squamous cell carcinoma.

ABSTRACT: BACKGROUND:TP53 gene mutations occur in more than 50% of human cancers and the vast majority of these mutations in human cancers are missense mutations, which broadly occur in DNA binding domain (DBD) (Amino acids 102-292) and mainly reside in six "hotspot" residues. TP53 G245C and R273H point mutations are two of the most frequent mutations in tumors and have been verified in several different cancers. In the previous study of the whole genome sequencing (WGS), we found some mutations of TP53 DBD in esophageal squamous cell carcinoma (ESCC) clinical samples. We focused on two high-frequent mutations TP53 p.G245C and TP53 p.R273H and investigated their oncogenic roles in ESCC cell lines, p53-defective cell lines H1299 and HCT116 p53-/-. RESULTS:MTS and colony formation assays showed that m

SUBMITTER: Kang N 

PROVIDER: S-EPMC6102840 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature