Effect of lyophilization on HRP-antibody conjugation: an enhanced antibody labeling technology.

ABSTRACT: OBJECTIVE:Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO). Conjugating enzyme with antibody without losing its enzymatic activity is a challenging task. Our aim is to modify existing classical method of conjugating antibodies with HRP to enhance immunoassay techniques with better sensitivity. We used chemicals such as sodium meta periodate to generate aldehyde group by oxidation of carbohydrate moieties on HRPO. The activated form of HRPO is lyophilized and then mixed with 1 mg/ml concentration of antibodies to be conjugate. RESULTS:After confirming chemical modification of conjugates via UV-Spec and SDS-PAGE independent molecules were used for conjugation and HRP-antibody conjugate. Fin

SUBMITTER: Regidi S 

PROVIDER: S-EPMC6102881 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature