Comprehensive high-resolution multiple-reaction monitoring mass spectrometry for targeted eicosanoid assays.

ABSTRACT: Eicosanoids comprise a class of bioactive lipids derived from a unique group of essential fatty acids that mediate a variety of important physiological functions. Owing to the structural diversity of these lipids, their analysis in biological samples is often a major challenge. Advancements in mass spectrometric have been helpful for the characterization and quantification of these molecular lipid species in complex matrices. However, there are technical limitations to this approach, including low-abundant and/or poorly ionizable lipids. Using high-resolution multiple-reaction monitoring (MRM^HR), we were able to develop a targeted bioanalytical method for eicosanoid quantification. For this, we optimized the LC-MS/MS conditions and evaluated several parameters, including lineari

SUBMITTER: Sorgi CA 

PROVIDER: S-EPMC6103261 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature