Lipid Structure and Composition Control Consequences of Interleaflet Coupling in Asymmetric Vesicles.

ABSTRACT: Using Förster resonance energy transfer, raft/liquid-ordered-domain formation was assessed in asymmetric vesicles containing outer leaflets composed of high-Tm (melting temperature) saturated phosphatidylcholines (diC_18:0PC, diC_16:0PC, diC_15:0PC, or diC_14:0PC), low-Tm unsaturated dioleoylphosphatidylcholine (DOPC) and cholesterol, and inner leaflets composed of lipids that by themselves would not form ordered domains (DOPC and cholesterol). Ordered-domain formation in the outer leaflet was compared to that in symmetric vesicles with the same lipid composition as the asymmetric vesicle outer leaflets. The difference between ordered-domain thermal stability in asymmetric and symmetric vesicles was highly dependent on high-Tm PC acyl-chain length. A

SUBMITTER: Wang Q 

PROVIDER: S-EPMC6103735 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature