Dynamic changes in ORC localization and replication fork progression during tissue differentiation.

ABSTRACT:

Background

Genomic regions repressed for DNA replication, resulting in either delayed replication in S phase or underreplication in polyploid cells, are thought to be controlled by inhibition of replication origin activation. Studies in Drosophila polytene cells, however, raised the possibility that impeding replication fork progression also plays a major role.

Results

We exploited genomic regions underreplicated (URs) with tissue specificity in Drosophila polytene cells to analyze mechanisms of replication repression. By localizing the Origin Recognition Complex (ORC) in the genome of the larval fat body and comparing this to ORC binding in the salivary gland, we found that sites of ORC binding show extensive tissue specificity. In contrast, there are common domains nearly

SUBMITTER: Hua BL 

PROVIDER: S-EPMC6103881 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature