The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling.

ABSTRACT: Genome-scale metabolic network models can be used for various analyses including the prediction of metabolic responses to changes in the environment. Legumes are well known for their rhizobial symbiosis that introduces nitrogen into the global nutrient cycle. Here, we describe a fully compartmentalised, mass and charge-balanced, genome-scale model of the clover Medicago truncatula, which has been adopted as a model organism for legumes. We employed flux balance analysis to demonstrate that the network is capable of producing biomass components in experimentally observed proportions, during day and night. By connecting the plant model to a model of its rhizobial symbiont, Sinorhizobium meliloti, we were able to investigate the effects of the symbiosis on metabolic fluxes and plant growth an

SUBMITTER: Pfau T 

PROVIDER: S-EPMC6104047 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature