Optimization of GFP Fluorescence Preservation by a Modified uDISCO Clearing Protocol.

ABSTRACT: Tissue optical clearing techniques provide alternative approaches for imaging large-volume specimens. uDISCO, an organic-solvent-based method, stands out from the enormous array of available optical clearing methods by achieving whole-brain imaging with high transparency, size reduction and fluorescence preservation. In this study, we aimed to modify the uDISCO protocol to achieve better fluorescence preservation and to thereby further improve its optical imaging quality. First, we determined the optimal pH value for optimized uDISCO, termed "a-uDISCO" (alkaline pH-based uDISCO). Then, we compared fluorescence preservation between a-uDISCO and uDISCO. In addition, we validated the clearing performance of the optimized method according to several parameters, including tissue transparency, s

SUBMITTER: Li Y 

PROVIDER: S-EPMC6104128 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature