The Culture Environment Influences Both Gene Regulation and Phenotypic Heterogeneity in Escherichia coli.

ABSTRACT: Microorganisms shape the composition of the medium they are growing in, which in turn has profound consequences on the reprogramming of the population gene-expression profile. In this paper, we investigate the progressive changes in pH and sugar availability in the medium of a growing Escherichia coli (E. coli) culture. We show how these changes have an effect on both the cellular heterogeneity within the microbial community and the gene-expression profile of the microbial population. We measure the changes in gene-expression as E. coli moves from lag, to exponential, and finally into stationary phase. We found that pathways linked to the changes in the medium composition such as ribosomal, tricarboxylic acid cycle (TCA), transport, and metabolism pathways are strongly

SUBMITTER: Smith A 

PROVIDER: S-EPMC6104134 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature