Quantitative RNA-seq Analysis Unveils Osmotic and Thermal Adaptation Mechanisms Relevant for Ectoine Production in Chromohalobacter salexigens.

ABSTRACT: Quantitative RNA sequencing (RNA-seq) and the complementary phenotypic assays were implemented to investigate the transcriptional responses of Chromohalobacter salexigens to osmotic and heat stress. These conditions trigger the synthesis of ectoine and hydroxyectoine, two compatible solutes of biotechnological interest. Our findings revealed that both stresses make a significant impact on C. salexigens global physiology. Apart from compatible solute metabolism, the most relevant adaptation mechanisms were related to "oxidative- and protein-folding- stress responses," "modulation of respiratory chain and related components," and "ion homeostasis." A general salt-dependent induction of genes related to the metabolism of ectoines, as well as repression of ectoine degradation gen

SUBMITTER: Salvador M 

PROVIDER: S-EPMC6104435 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature