Association of cardiac myosin-binding protein-C with the ryanodine receptor channel - putative retrograde regulation?

ABSTRACT: The cardiac muscle ryanodine receptor-Ca²⁺ release channel (RyR2) constitutes the sarcoplasmic reticulum (SR) Ca²⁺ efflux mechanism that initiates myocyte contraction, while cardiac myosin-binding protein-C (cMyBP-C; also known as MYBPC3) mediates regulation of acto-myosin cross-bridge cycling. In this paper, we provide the first evidence for the presence of direct interaction between these two proteins, forming a RyR2-cMyBP-C complex. The C-terminus of cMyBP-C binds with the RyR2 N-terminus in mammalian cells and the interaction is not mediated by a fibronectin-like domain. Notably, we detected complex formation between both recombinant cMyBP-C and RyR2, as well as between the native proteins in cardiac tissue. Cellular Ca²⁺ dynamics in HEK293 cells is alt

SUBMITTER: Stanczyk PJ 

PROVIDER: S-EPMC6104826 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature