A novel live-cell imaging system reveals a reversible hydrostatic pressure impact on cell-cycle progression.

ABSTRACT: Life is dependent upon the ability of a cell to rapidly respond to changes in the environment. Small perturbations in local environments change the ability of molecules to interact and, hence, communicate. Hydrostatic pressure provides a rapid non-invasive, fully reversible method for modulating affinities between molecules both in vivo and in vitro We have developed a simple fluorescence imaging chamber that allows intracellular protein dynamics and molecular events to be followed at pressures <200 bar in living cells. By using yeast, we investigated the impact of hydrostatic pressure upon cell growth and cell-cycle progression. While 100 bar has no effect upon viability, it induces a delay in chromosome segregation, resulting in the accumulation of long undivided cells that

SUBMITTER: Brooker HR 

PROVIDER: S-EPMC6104828 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature