Transcriptome analysis of adult Caenorhabditis elegans cells reveals tissue-specific gene and isoform expression.

ABSTRACT: The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans' four major tissues (or "tissue-ome"), identifying ubiquitously expressed and tissue-specific "enriched" genes. These data newly reveal the hypodermis' metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms. Finally, w

SUBMITTER: Kaletsky R 

PROVIDER: S-EPMC6105014 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature