Immunohistochemical Identification of Human Skeletal Muscle Macrophages.

ABSTRACT: Macrophages have well-characterized roles in skeletal muscle repair and regeneration. Relatively little is known regarding the role of resident macrophages in skeletal muscle homeostasis, extracellular matrix remodeling, growth, metabolism and adaptation to various stimuli including exercise and training. Despite speculation into macrophage contributions during these processes, studies characterizing macrophages in non-injured muscle are limited and methods used to identify macrophages vary. A standardized method for the identification of human resident skeletal muscle macrophages will aide in the characterization of these immune cells and allow for the comparison of results across studies. Here, we present an immunohistochemistry (IHC) protocol, validated by flow cytometry, to distinctly

SUBMITTER: Kosmac K 

PROVIDER: S-EPMC6105281 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature