Integral bHLH factor regulation of cell cycle exit and RGC differentiation.

ABSTRACT:

Background

In the developing mouse embryo, the bHLH transcription factor Neurog2 is transiently expressed by retinal progenitor cells and required for the initial wave of neurogenesis. Remarkably, another bHLH factor, Ascl1, normally not present in the embryonic Neurog2 retinal lineage, can rescue the temporal phenotypes of Neurog2 mutants.

Results

Here we show that Neurog2 simultaneously promotes terminal cell cycle exit and retinal ganglion cell differentiation, using mitotic window labeling and integrating these results with retinal marker quantifications. We also analyzed the transcriptomes of E12.5 GFP-expressing cells from Neurog2^GFP/+ , Neurog2^GFP/GFP , and Neurog2^Ascl1KI/GFP eyes, and validated the most significantly affected genes us

SUBMITTER: Maurer KA 

PROVIDER: S-EPMC6105502 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature