Identification of Mycobacterial Ribosomal Proteins as Targets for CD4⁺ T Cells That Enhance Protective Immunity in Tuberculosis.

ABSTRACT: Mycobacterium tuberculosis remains a threat to global health, and a more efficacious vaccine is needed to prevent disease caused by M. tuberculosis We previously reported that the mycobacterial ribosome is a major target of CD4⁺ T cells in mice immunized with a genetically modified Mycobacterium smegmatis strain (IKEPLUS) but not in mice immunized with Mycobacterium bovis BCG. Two specific ribosomal proteins, RplJ and RpsA, were identified as cross-reactive targets of M. tuberculosis, but the breadth of the CD4⁺ T cell response to M. tuberculosis ribosomes was not determined. In the present study, a library of M. tuberculosis ribosomal proteins and in silico-predicted peptide libraries were used to screen CD4

SUBMITTER: Kennedy SC 

PROVIDER: S-EPMC6105890 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature