IL-6-mediated cross-talk between human preadipocytes and ductal carcinoma in situ in breast cancer progression.

ABSTRACT:

Background

The function of preadipocytes in the progression of early stage breast cancer has not been fully elucidated at the molecular level. To delineate the role of preadipocytes in breast cancer progression, we investigated the cross-talk between human breast ductal carcinoma in situ (DCIS) cells and preadipocytes with both an in vitro culture and xenograft tumor model.

Methods

GFP or RFP was transduced into human DCIS cell line MCF10DCIS.com cells or preadipocytes using lentivirus. Cell sorter was used to separate pure, viable populations of GFP- or RFP-transduced cells. Cell viability and proliferation was assessed by crystal violet assays and cell migration and invasion capability was assayed by the transwell strategy. Gene and protein levels were measured by western

SUBMITTER: Kim HS 

PROVIDER: S-EPMC6106749 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature