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Add-on plasmonic patch as a universal fluorescence enhancer.

ABSTRACT: Fluorescence-based techniques are the cornerstone of modern biomedical optics, with applications ranging from bioimaging at various scales (organelle to organism) to detection and quantification of a wide variety of biological species of interest. However, the weakness of the fluorescence signal remains a persistent challenge in meeting the ever-increasing demand to image, detect, and quantify biological species with low abundance. Here, we report a simple and universal method based on a flexible and conformal elastomeric film with adsorbed plasmonic nanostructures, which we term a "plasmonic patch," that provides large (up to 100-fold) and uniform fluorescence enhancement on a variety of surfaces through simple transfer of the plasmonic patch to the surface. We demonstrate the applications of the plasmonic patch in improving the sensitivity and limit of detection (by more than 100 times) of fluorescence-based immunoassays implemented in microtiter plates and in microarray format. The novel fluorescence enhancement approach presented here represents a disease, biomarker, and application agnostic ubiquitously applicable fundamental and enabling technology to immediately improve the sensitivity of existing analytical methodologies in an easy-to-handle and cost-effective manner, without changing the original procedures of the existing techniques.

SUBMITTER: Luan J

PROVIDER: S-EPMC6107004 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Add-on plasmonic patch as a universal fluorescence enhancer.

Luan Jingyi J Morrissey Jeremiah J JJ Wang Zheyu Z Derami Hamed Gholami HG Liu Keng-Ku KK Cao Sisi S Jiang Qisheng Q Wang Congzhou C Kharasch Evan D ED Naik Rajesh R RR Singamaneni Srikanth S

Light, science & applications 20180704

Fluorescence-based techniques are the cornerstone of modern biomedical optics, with applications ranging from bioimaging at various scales (organelle to organism) to detection and quantification of a wide variety of biological species of interest. However, the weakness of the fluorescence signal remains a persistent challenge in meeting the ever-increasing demand to image, detect, and quantify biological species with low abundance. Here, we report a simple and universal method based on a flexibl ...[more]

PMID: 30839611

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Project description:Fluorescence technology pervades all areas of chemical and biological sciences. In recent years, it is being realized that traditional fluorescence can be enriched in many ways by harnessing the power of plasmonic or photonic structures that have remarkable abilities to mold the flow of optical energy. Conventional fluorescence is omnidirectional in nature, which makes it difficult to capture the entire emission. Suitably designed emission directivity can improve collection efficiency and is desirable for many fluorescence-based applications like sensing, imaging, single molecule spectroscopy, and optical communication. By incorporating fluorophores in plasmonic or photonic substrates, it is possible to tailor the optical environment surrounding the fluorophores and to modify the spatial distribution of emission. This promising approach works on the principle of near-field interaction of fluorescence with spectrally overlapping optical modes present in the substrates. In this Account, we present our studies on directional emission with different kinds of planar metallic, dielectric, and hybrid structures. In metal-dielectric substrates, the coupling of fluorescence with surface plasmons leads to directional surface-plasmon-coupled emission with characteristic dispersion and polarization properties. In one-dimensional photonic crystals (1DPC), fluorophores can interact with Bloch surface waves, giving rise to sharply directional Bloch surface wave-coupled emission. The interaction of fluorescence with Fabry-Pérot-like modes in metal-dielectric-metal substrates and with Tamm states in plasmonic-photonic hybrid substrates provides beaming emission normal to the substrate surface. These interesting features are explained in the context of reflectivity dispersion diagrams, which provide a complete picture of the mode profiles and the corresponding coupled emission patterns. Other than planar substrates, specially fabricated plasmonic nanoantennas also have tremendous potential in controlling and steering fluorescence beams. Some representative studies by other research groups with various nanoantenna structures are described. While there are complexities to near-field interactions of fluorescence with plasmonic and photonic structures, there are also many exciting possibilities. The routing of each emission wavelength along a specific direction with a given angular width and polarization will allow spatial and spectral multiplexing. Directional emission close to surface normal will be particularly useful for microscopy and array-based studies. Application-specific angular emission patterns can be obtained by varying the design parameters of the plasmonic/photonic substrates in a flexible manner. We anticipate that the ability to control the flow of emitted light in the nanoscale will lead to the development of a new generation of fluorescence-based assays, instrumentation, portable diagnostics, and emissive devices.

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Add-on plasmonic patch as a universal fluorescence enhancer.

Publications

Add-on plasmonic patch as a universal fluorescence enhancer.

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