Label-free 3D computational imaging of spermatozoon locomotion, head spin and flagellum beating over a large volume.

ABSTRACT: We report a high-throughput and label-free computational imaging technique that simultaneously measures in three-dimensional (3D) space the locomotion and angular spin of the freely moving heads of microswimmers and the beating patterns of their flagella over a sample volume more than two orders-of-magnitude larger compared to existing optical modalities. Using this platform, we quantified the 3D locomotion of 2133 bovine sperms and determined the spin axis and the angular velocity of the sperm head, providing the perspective of an observer seated at the moving and spinning sperm head. In this constantly transforming perspective, flagellum-beating patterns are decoupled from both the 3D translation and spin of the head, which provides the opportunity to truly investigate the 3D spatio-temp

SUBMITTER: Daloglu MU 

PROVIDER: S-EPMC6107047 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature