Glutamate receptor δ2 serum antibodies in pediatric opsoclonus myoclonus ataxia syndrome.

ABSTRACT:

Objective

To identify neuronal surface antibodies in opsoclonus myoclonus ataxia syndrome (OMAS) using contemporary antigen discovery methodology.

Methods

OMAS patient serum immunoglobulin G immunohistochemistry using age-equivalent rat cerebellar tissue was followed by immunoprecipitation, gel electrophoresis, and mass spectrometry. Data are available via ProteomeXchange (identifier PXD009578). This generated a list of potential neuronal surface cerebellar autoantigens. Live cell-based assays were used to confirm membrane-surface antigens and adsorb antigen-specific immunoglobulin Gs. The serologic results were compared to the clinical data.

Results

Four of the 6 OMAS sera tested bound rat cerebellar sections. Two of these sera with similar immunoreactivities were us

SUBMITTER: Berridge G 

PROVIDER: S-EPMC6107266 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature