CD4⁺ and CD8⁺ T-Cell-Specific DNA Cytosine Methylation Differences Associated With Obesity.

ABSTRACT:

Objective

Lifestyle factors associated with obesity may alter epigenome-regulated gene expression. Most studies examining epigenetic changes in obesity have analyzed DNA 5´-methylcytosine (5mC) in whole blood, representing a weighted average of several distantly related and regulated leukocyte classes. To examine leukocyte-specific differences associated with obesity, a pilot study examining 5mC in three distinct leukocyte types isolated from peripheral blood of women with normal weight and obesity was conducted.

Methods

CD4⁺ T cells, CD8⁺ T cells, and CD16⁺ neutrophils were reiteratively isolated from blood, and 5mC levels were measured across >450,000 CG sites.

Results

Nineteen CG sites were differentially methylated between women wi

SUBMITTER: Hohos NM 

PROVIDER: S-EPMC6107382 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature