Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs.

ABSTRACT: Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we demonstrate the functional reconstitution of a large soluble-domain containing positively-charged ∼57 kDa cytochrome-P450 and negatively-charged ∼16 kDa cytochrome-b5 in lipid-nanodiscs, and the role of the polymer charge for high-resolution studies on membrane proteins.

SUBMITTER: Ravula T 

PROVIDER: S-EPMC6107395 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature