Genes encoding membrane proteins showed stable expression in rice under aerobic condition: novel set of reference genes for expression studies.

ABSTRACT: A set of reference genes expressing stably under aerobic and anaerobic conditions in rice is essential to execute omics studies relating to aerobic adaptations. Stability of expression of ten rice reference genes, viz. Actin, eEF-1a, eIF-5C, Exp1, Exp2, Memp, SKP1A, TF-SUI1, TPH, and UBQ5 was validated across six experimental sets in shoot and root tissues at seedling, tillering, and panicle initiation stages. Comprehensively, Memp (Membrane protein), TPH (Tumor protein homolog), and Exp1 (Expressed protein) were revealed as the most stable set with acceptable M and V value according to the gold standards of qRT-PCR using various algorithms/tools. The identified set of reference genes was validated using root trait genes, which showed concur

SUBMITTER: Phule AS 

PROVIDER: S-EPMC6107477 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature